Withania Somnifera L Root Extract Ameliorates Toxin Induced Cytotoxicity

In this study the use of plants for medicinal purpose used locally in the treatment of various diseases and we examined for their antioxidant activity. Therefore, the present investigation is part of continuing programme related to the biochemical screening of local plants used in Ancient Indian Medicine, Ayurveda, Siddha and Yunani. An Aqueous root extract of Withania Somnifera (L.) Dunal (Ashwagandha) was evaluated for its protective effect (antioxidant effect) against Cypermethrin (CM) toxin induced oxidation in male albino rats. Cypermethrin, (CM) toxin [(RS)-3-phenoxybenzyl (IRS)-cis-trans-3-(2,2-dichlorovinyl)-2,2-dimethyl cyclopropane carboxylate] a Type II pyrethroid pesticide is commonly used in agriculture and many other domestic applications for pest control. Studies have shown that cypermethrin (toxin) induces lipid peroxidation and alters the antioxidant status in non-target organisms. Aqueous root extract of Withania Somnifera (L.) Dunal (Ashwagandha), a source of several flavonoids and steroids is a potent antioxidant. In our present study, an attempt has been made to study the effect of Cypermethrin (CM) toxin on biochemical parameters and ameliorating effect of Withania Somnifera (L.) Dunal (Ashwagandha) in male wistar rats. Adult male wistar rats were divided into four different groups. Group I Served as vehicle treated normal saline (Control), Group II Rats received intragastric tubing (Ig) injection of cypermethrin (CM) toxin1/10 LD50 (LD50 of CM is 250mg) (25mg/kgbw/day in corn oil), Group III received Withania Somnifera (10%) aqueous root extract (5ml of 10% extract per day, for 60 days) Group IV Rats received Withania Somnifera aqueous root extract alone (5ml of 10% per day for 60 days) treated. Cypermethirn (CM) toxin induction leads to reduction in the levels of Enzymic and Non-Enzymic antioxidants levels. However, on treatment with Withania Somnifera aqueous root extract normalized the levels of all the biochemical and hematological parameters. These findings highlight the efficacy of Withania Somnifera aqueous root extract as protective effects Cypermethirn (CM) toxin induced oxidative stress.